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Hyperinsulinemia and Oxidative Stress
P. KYSELOVÁ, M. ŽOUREK, Z. RUŠAVÝ, L. TREFIL1,
J. RACEK1
First Department of Internal Medicine and 1Department
of Clinical and Experimental Biochemistry, University Hospital,
Pilsen, Czech Republic
Received November 29,
2001
Accepted April 12, 2002
Summary
The aim of the study was to compare the effect of short-term
hyperglycemia and short-term hyperinsulinemia on parameters of
oxidative stress in Wistar rats. Twenty male rats (aged 3
months, average body weight 325 g) were tested by
hyperinsulinemic clamp (100 IU/l) at two different glycemia
levels (6 and 12 mmol/l). Further 20 rats were used as a control
group infused with normal saline (instead of insulin) and 30 %
glucose simultaneously. Measured parameters of oxidative stress
were malondialdehyde (MDA), reduced glutathione (GSH) and total
antioxidant capacity (AOC). AOC remained unchanged during
hyperglycemia and hyperinsulinemia. Malondialdehyde (as a marker
of lipid peroxidation) decreased significantly (p<0.05) during
the euglycemic hyperinsulinemic clamp, and increased
significantly during isolated hyperglycemia without
hyperinsulinemia. Reduced glutathione decreased significantly
(p<0.05) during hyperglycemia without hyperinsulinemia. These
results suggest that the short-term exogenous hyperinsulinemia
reduced the production of reactive oxygen species (ROS) during
hyperglycemia in an animal model compared with the control
group.
Key
words
Oxidative stress ·
Hyperglycemia
· Hyperinsulinemia
· Rat
Reprint
requests
Dr. P. Kyselová, Department of Internal Medicine, University
Hospital, Alej Svobody 80, 304 60 Pilsen, Czech Republic.
E-mail: Kyselova@fnplzen.cz
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