| Protective Effects
of Topical Alpha-Tocopherol Acetate on UVB
Irradiation in Guinea Pigs: Importance of Free
Radicals
Y.
SARAL, B. UYAR, A. AYAR1, M. NAZIROGLU2
Firat
University, Faculty of Medicine, Department of
Dermatology, 1Department
of Pharmacology, 2Department
of Physiology, Faculty of Veterinary Medicine,
Elazig, Turkey
Received April 14, 2001
Accepted October 5, 2001
Summary
Reactive oxygen species can be generated
by daily exposure of the skin to ultraviolet
light and may cause some subchronic and chronic
skin disorders. The aim of this study was to
investigate a possible preventive role of
a-tocopherol acetate (ATA) on ultraviolet B (UVB)
induced peroxidation by assessing lipid peroxide
(LPO) levels and activity of reactive oxygen
scavenging enzymes including glutathione
peroxidase and superoxide dismutase (SOD) in
guinea pigs. ATA was topically applied to the
skin for three weeks before a single dose of 0.9
J/cm2 UVB irradiation on the skin and lipid
peroxide levels and antioxidants in plasma, skin
and liver and erythrocytes were determined after
decapitation. Topical application of ATA
prevented the UVB irradiation-induced reduction
of scavenging enzyme activities in skin and
erythrocytes. In conclusion, we suggest that
topical applications of ATA before UVB
irradiation is effective in protecting the skin
from unwanted effects of UVB irradiation.
.
Key
words
Alpha-tocopherol
· Antioxidants · Guinea pig · Lipid peroxide
· Skin · Ultraviolet B
Reprint
requests
Dr. Ahmet AYAR, Department of Pharmacology, Firat
University, Faculty of Medicine (Tip Fakultesi),
TR-23119 Elazig, Turkey. Fax: + (90) 424 237 91
38, e-mail: aayar@firat.edu.tr
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