Template:ITSx (Extract ITS)
ITSx (Extract ITS)
To extract various parts of fungal ribosomal DNA using an external program ITSx click on ITSx (Extract ITS) in Extraction menu of FASTA form menu bar. Choose (check) region(s) to be cut and number of threads (CPUs) to be utilized for calculation (a maximum is given by type of your computer processor). Chosen region(s) will be extracted from currently opened (viewed) dataset.
After you click Extract ITS button you will be prompted to define output location and name an output file.
Note: Multiple files will be created under the output file name. We recommend creating a specific output folder in desired location and saving output file there.
When the extraction is finished click OK and following window with summary of results appears.
The output files should be located in the previously specified folder. The extracted regions shall be in the respective fasta file (E.g. Extracted ITS2 sequences in *.ITS2.fasta).